Journal:

Article Title: TeiR, a LuxR-Type Transcription Factor Required for Testosterone Degradation in Comamonas testosteroni

doi: 10.1128/JB.186.5.1430-1437.2004

Figure Lengend Snippet: Bacterial strains, plasmids, and cosmids used

Article Snippet: pGEMT easy , Ap r ; PCR fragment cloning vector , Promega.

Techniques: Mutagenesis, Clone Assay, Plasmid Preparation

Journal:

Article Title: Identification and Characterization of Two Chemotactic Transducers for Inorganic Phosphate in Pseudomonas aeruginosa

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: For P i limitation, P. aeruginosa cells grown overnight in 2× YT medium were inoculated (a 2.5% inoculum) into T 0 medium, which was prepared by omitting P i from T 5 medium, and incubated at 37°C with shaking. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristics Reference or source E. coli MV1184 ara Δ( lac-proAB ) rpsL thi (φ80 lacZ ΔM15) Δ( srl-recA ) 306 ::Tn 10 (Tc r ) F′[ traD36 proAB + lacl q lacZ ΔM15] 23 HB101 hsdS20 recA13 proA2 leu6 thi-1 rpsL20 ara-14 galK2 lacY1 xyl-5 mtl-1 supE44 3 P. aeruginosa PAO1 Prototroph, FP − (sex factor minus) 8 PP1 PAO1 derivative, ctpH :: kan This study PP2 PAO1 derivative, ctpL :: kan This study PP3 PAO1 derivative, ctpH :: tet ctpL :: kan This study APC1 PAO1 derivative, phoU 12 PHOB1 PAO1 derivative, phoB :: kan 12 PP4 PAO1 derivative, phoU ctpH :: kan This study PP5 PAO1 derivative, phoU ctpL :: kan This study PP6 PAO1 derivative, phoB :: kan ctpH :: tet This study PP7 PAO1 derivative, phoB :: kan ctpL :: tet This study Plasmids pGEM-T Easy PCR cloning vector; Ap r lacPOZ ′ Promega pUC118Tc pUC118 containing a 1.3-kb tet cartridge from pBR322; Ap r Tc r This study pUC4K pUC4 containing a 1.3-kb kan cartridge; Ap r Km r Pharmacia pCP19 Broad-host-range cosmid; Tc r IncP 6 pPT10.1 pGEM-T Easy with a 3.6-kb PCR fragment containing ctpH ; Ap r This study pPT11.1 pGEM-T Easy with a 3.3-kb PCR fragment containing ctpL ; Ap r This study pPT10.2 pPT10.1 derivative containing a 1.3-kb kan cartridge This study pPT11.2 pPT11.1 derivative containing a 1.3-kb kan cartridge This study pPT10.3 pPT10.1 derivative containing a 1.3-kb tet cartridge This study pPT10.4 pCP19 derivative containing a 3.6-kb Eco RI fragment of pPT10.1; Tc r This study pPT11.4 pCP19 derivative containing a 2.3-kb Eco RV fragment of pPT11.1; Tc r This study pKZ27 Broad-host-range transcriptional fusion vector; Km r lacZ IncQ This study pKZ27.1 pKZ27 containing a 1.2-kb fragment with amp gene from pBR322; Km r Cb r This study pPT10.5 pKZ27 derivative containing a 1.8-kb Kpn I fragment of pPT10.1; Km r This study pPT10.6 pKZ27.1 derivative containing a 1.8-kb Kpn I fragment of pPT10.1; Km r Cb r This study pPT11.5 pKZ27 derivative containing a 1.6-kb Eco RV- Sal I fragment of pPT10.1; Km r This study pPT11.6 pKZ27.1 derivative containing a 1.6-kb Eco RV- Sal I fragment of pPT10.1; Km r Cb r This study Open in a separate window Bacterial strains and plasmids used in this study Chemotaxis assays.

Techniques: Plasmid Preparation, PCR Cloning

Journal:

Article Title: Critical Role of Multidrug Efflux Pump CmeABC in Bile Resistance and In Vivo Colonization of Campylobacter jejuni

doi: 10.1128/IAI.71.8.4250-4259.2003

Figure Lengend Snippet: Bacterial plasmids and strains used in this study

Article Snippet: When needed, culture media were supplemented with kanamycin (30 μg/ml) or chloramphenicol (20 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Plasmid or strain Description Source or reference Plasmids pGEMT-Easy PCR cloning vector, Amp r Promega pCMEC pGEMT-Easy containing 1.5-kb cmeC fragment, Amp r This study pCMECK pCMEC with kanamycin resistance cassette inserted in cmeC gene, Amp r Kan r This study pUOA18 E. coli-C. jejuni shuttle vector, Cm r 49 pCME pUOA18 derivative containing a wild-type cmeABC operon This study Strains C. jejuni 81-176 Wild type; isolated from human 7 21190 Wild type; isolated from chicken 25 JL101 21190 derivative; cmeB :: kan 28 JL102 21190 derivative; cmeC :: kan This study JL103 JL101/pCME, Kan r Cm r This study JL104 JL102/pCME, Kan r Cm r This study E. coli JM109 endA1 recA1 gyrA96 thi hsdR17 (r k − m k + ) relA1 supE44 Δ( lac-proAB ) [F′ traD36 lac I q Z ΔM15] Promega Open in a separate window Bacterial plasmids and strains used in this study

Techniques: PCR Cloning, Plasmid Preparation, Isolation